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. 2011 Jan 18;6(1):e14514. doi: 10.1371/journal.pone.0014514

Figure 4. Cyclin D2 level is down-regulated with OTUD-6B overexpression.

Figure 4

A & B & C. Real-time PCR, RT-PCR, and immunoblot of OTUD-6B, GAPDH, cyclin D1, cyclin D2, cyclin D3, p21, p27, p15, p16, cdk4, cdk6, cdc2, cyclin E, Rb, and c-Myc genes from total RNA samples and protein extracts of DOX(−) and DOX(+) Hela cells. Hela cells were provided by the Cell Bank, Shanghai Institute of Biochemistry and Cell Biology, SIBS, CAS. D & E. Immunoblot of OTUD-6B, cyclin D2, and p27 with gradient concentrations (0, 0.1, 0.5, 1, 2 ug/ml) of DOX stimulation. Quantitation was performed with Bandscan 5.0 from 3 independent results. F & G. Immunoblot (left panel) for cyclin D1, cyclin D2, and cyclin D3 on Hela and Ba/F3 cells overexpressing pcDNA3.1(+), HA-OTUD-6B WT, and HA-OTUD-6B C188S. Quantitation (right panel) of cyclin D1, cyclin D2, and cyclin D3 on Hela and Ba/F3 cells was performed with Bandscan 5.0 from three independent results.