Figure 4. Cell death phenotypes and JNK activation caused by hppy and Tor.

(A–B) Expression of activated Cas3 (green) and FasIII (red) in nub-Gal4 UAS-hppy/+ (A) and ap-Gal4 UAS-hppy/+ (B) third instar wing discs. (C–D) The presence of apoptotic cells is suppressed when DIAP (C) or Puc (D) are over-expressed with hppy. Below A and C are the corresponding Z sections of the discs. In C the nuclei are labelled by Topro (red). (E) Expression of βGal (red) and GFP (green) in nub-Gal4 UAS-GFP/UAS-hppy; puc-lacZ/+. (F) Expression of βGal (red) and GFP (green) in en-Gal4 UAS-GFP/UAS-hppy; puc-lacZ/+. In both cases there is a strong induction of puc expression in the domain of hppy over-expression (red channels in E' and F'). (G–G') Expression of puc-lacZ (red) in en-gal4 UAS-GFP/UAS-ihppy; puc-lacZ/+ third instar disc dissected 8 hours after irradiation. The image is a projection of 12 focal planes through the wing disc epithelium. G' shows the corresponding red channel. (H) Ratio between the intensity of the red signal (puc-lacZ expression) between the anterior and posterior compartments of en-Gal4 UAS-GFP/puc-lacZ (control, black column) and en-Gal4 UAS-GFP/puc-lacZ; UAS-ihppy/+ (i-happy, red column). The value of the t-student test is also indicated (t: 0.002). (I–J) Induction of cell death in en-Gal4 UAS-GFP/UAS-Tor (I) and en-Gal4 UAS-GFP/ UAS-Tor^TED (J) wing discs. The expression of activated Cas3 is in red and GFP in green. The arrowhead points to the places where activated Cas3 is detected.