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. 2010 Dec 24;10:392. doi: 10.1186/1471-2148-10-392

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Copyright ©2010 Alquezar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ITS2 genotypes of A. longirostris. Panel A: ITS2 PCR products run through a 1.0% agarose gel indicate some small size variation. Panel B: The same ITS2 PCR products cut with Msp I and run through a 3.0% agarose gel reveals eight distinct RFLP genotype profiles. Panel C: Most ITS2-RFLP genotypes revealed the same heteroduplex profile when PCR products were run through a 7.0% acrylamide gel suggesting copy variants are fixed within individuals and within interbreeding populations. However, genotype C contained two distinct heteroduplex profiles (designated C1 and C2) within its RFLP profile revealing the presence of two independently evolving ITS2 genotypes.