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. 2010 Dec 24;10:392. doi: 10.1186/1471-2148-10-392

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Copyright ©2010 Alquezar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phylogenetic assessment of A. longirostris based on DNA sequence from the ITS2 (Panel A) and mtDNA COI (Panel B) reveal nine lineages. Both Bayesian and Maximum Likelihood procedures produced trees of similar topology with branch support values above 70% shown - Bayesian posterior probability above the branch (converted to percentage) and Maximum Likelihood bootstrapping (percentage) below the branch. The ITS2 showed a well resolved tree with all clades well separated into genotypes. All ITS2 genotypes were evident as mtDNA COI divergent lineages with cloned individuals co- assessed for the COI.