Table 2.
Primers used in this study.
| Target gene | Primer sequences (5' → 3') | Amplicon size (bp) | Application |
|---|---|---|---|
| ef1415 | F:TGTTGCGGTTTCTGCATTAG | 2818 | PCR on junction between EF1415 and EF1417 |
| ef1417 | R:GCATCTCGATAGACAATTCG | PCR on junction between EF1415 and EF1417 | |
| ef1489 | F:GAATCGAACTAGCATTTTTGGG | 465 | PCR on junction between EF1489 and EF1490 |
| ef1490 | R:ATGGAACGAACCATTGGAAA | PCR on junction between EF1489 and EF1490 | |
| ef1843 | F:GGAGCCGTTAGACAGACAGC | 2457 | PCR on junction between EF1843 and EF1847 |
| ef1847 | R:GCTTGCTTTACAGCCTCAAGA | PCR on junction between EF1843 and EF1847 | |
| ef1895 | F:GCACAACAAATTTCAATTCCA | 4573 | PCR on junction between EF1895 and EF1898 |
| ef1898 | R:ATTGAAGTGGTTCGCTACGG | PCR on junction between EF1895 and EF1898 | |
| ef2239 | F:AACTGCTGTCAAGCGTAGCA | 1252 | PCR on junction between EF2239 and EF2240 |
| ef2240 | R:TGTGGCATTTTGGACTGTTG | PCR on junction between EF2239 and EF2240 | |
| ef2350 | F:ATAACTGAGTGATTTTCACAATTGC | 654 | PCR on junction between EF2350 and EF2352 |
| ef2352 | R:GATCCGTGGAAGTTCCTCAA | PCR on junction between EF2350 and EF2352 | |
| ef3216 | F:TCGGCGTTGAAGACTATGAA | - | Sequencing of junction between EF3216 and EF3230 |
| ef3217 | F:ATTGGGAATGACGGCTACAC R:TTGCGTATTTCGCAGCATAA |
499 | PCR |
| ef3218 | F:TCGCGTAGTAGGAGCAATCA R:TTTTGTTCAGTTCCCACACCT |
396 | PCR |
| ef3220 | F:AGCTTTTGGCGAAGGAGATT R:TTTATTGCGGGTTCCTCAGT |
495 | PCR |
| ef3221 | F:TGAACGAAAATGAAGGTGGT R:TCATCAATCTCCAACGCATC |
196 | PCR |
| ef3222 | F:CAAAGAAGAATCAGCCGATTAAA R:ATATTTGGGCATTTGCATGG |
183 | PCR |
| ef3223 | F:AATTGGGAAAAAGGGGTCAG R:TTCGTGATCTGCTTGTTGTTCT |
501 | PCR |
| ef3224 | F:GTTGGGCTGGACGTATGAAT R:TGTGGCTTTATAGGCTGTAGCA |
214 | PCR |
| ef3225 | F:ATTACTTCACCGCCCATGAC R:CGCTGGAAGTCTGCTCTTG |
474 | PCR |
| ef3226 | F:GATGATTTAACCGCACAAGGA R:TTTTTATTTCGAGCGGATGC |
499 | PCR |
| ef3227 | F:ACAGGAAGCCATTCACAAACT R:CTGATTCGTGGAAGTCCAACT |
162 | PCR |
| ef3230 | R:TCCTGACTTCCGTTCTGCTT | - | Sequencing of junction between EF3216 and EF3230 |
| hmpref0346_1861 | F:CGAGTTAGAGGAAGCGTTGG | 630 | PCR |
| R:CCAGACAATTTGGGCGTACT | |||
| hmpref0346_1864 | F:GAAATTTTCTGAAAGTGAAGACAAGA | 299 | PCR |
| R:TGATTAGCAGTCACAACAGCAA | |||
| hmpref0346_1868 | F:TGTACACAAGCTACCCGGATT | 538 | PCR |
| R:TTCCCACCTGCGTCTATTTT | |||
| hmpref0348_0427 | R:GAGACTTCAACCACTCCACAAAAACC | - | Sequencing of gap between contig00034-35 in TX0104 |
| hmpref0348_0428 | F:CCTGTAGAAGTATTGTCCATTTTAACGCTATC | Sequencing of gap between contig00034-35 in TX0104 |