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. 2011 Jan 6;11:4. doi: 10.1186/1471-2180-11-4

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Copyright ©2011 Luginbuehl et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of TbrPPX1. Panels A-C: procyclic forms. Panels D-F: bloodstream forms. Panels A and D: c-Myc-tagged TbrPPX1; panels B and E: acidocalcisomes visualized by the VH⁺-PPase-antibody; panels C and F: overlay, including DAPI staining. Panel G: Detergent fractionation of bloodstream forms and procyclic cells. Pellets (P) and supernatant fractions (SN) of cells solubilized either with RIPA buffer or with 0.5% Triton X-100. Western blots were developed with monoclonal anti-c-Myc antibody (= TbrPPX1), a polyclonal antiserum against BIP, and a polyclonal antiserum against a major paraflagellar rod (PFR) protein.