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. 2011 Jan 18;6(1):e15879. doi: 10.1371/journal.pone.0015879

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Rajoria et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole cell protein (20 µg) was resolved by SDS-PAGE followed by Western blot analysis for ER-α (dilution 1∶500), ER-β (dilution 1∶1000) and actin (dilution 1∶5000). All the cell lines used in this study (BCPAP, 8505C, CGTHW-1 and ML-1) express both ER-α and ER-β at comparable levels.