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. 2010 Dec 31;11:50. doi: 10.1186/1471-2091-11-50

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Copyright ©2010 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Single step fluorescent detection of SBP-tagged SMC2. Cyto-centrifuged DT40 wild-type cells (A) and SMC2-SBP cells (B, C) were incubated with streptavidin-488 for single-step fluorescence analysis. Mitotic chromosomes from DT40 wild-type cells show no staining, whilst chromosomes from SMC2-SBP display the distinct characteristic chromosome scaffold staining pattern of condensin subunits. Note also in (C) that the interphase nuclei display weak diffuse staining of SMC2-SBP cells which is also consistent with known condensin antibody staining [8].