Figure 4.

Effects of media on vtPA production in the cytoplasm of E. coli. Panel A) Relative fold activity of vtPA produced in EnBase media at 30°C, normalized to the equivalent of the best previously reported system (rosetta-gami with co-expression of mature DsbC) and shown as mean ± s.d. (n = 2-6; see additional file 1: Table of vtPA production and activity data). Strains: BL = BL21 (DE3) pLysSRARE; RG = rosetta-gami; OG = origami. For Erv1p and mature DsbC expression + = co-expression while +p = pre-expression. Panel B) Relative yields of active vtPA made as an MBP fusion protein grown in LB and EnBase media in shake flasks at 30°C. Nomenclature as per the previous panel.