Figure 1.

Prevention of poly (I:C) induced activation of an IFN-β promoter by the NS1 protein in A549 cells. (A) Forty-eight hours after transfection, the cells were harvested and assayed for luciferase activity. Average relative luciferase activities are reported. Data are expressed as the mean ± S.E. for the three independent experiments performed in duplicate. (B)Western blotting was performed to compare level of expression of the two NS1 constructs. Expression of the NS1 proteins in A549 cells transfected with the NS1 constructs; pNS-chicken/49 and pNS-mink/84, was confirmed at 0, 2, 4, 8, 16 and 24 hours post transfection.