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. 2010 Dec 15;10:321. doi: 10.1186/1471-2180-10-321

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Copyright ©2010 Kato et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Specific activities of the quinone-dependent D-lactate dehydrogenase Dld (A) and growth (B) of various C. glutamicum strains. Specific Dld activities (A) were determined after growth in LB complex medium containing 1 mM IPTG. The values represent means and standard deviations of at least three independent cultivations. Growth (B) of C. glutamicum WT(pEKEx3) (diamonds), WT(pEKEx3-dld) (circles), ::dld(pEKEx3) (squares), and ::dld(pEKEx3-dld) (triangles) in CgXII mineral medium containing 100 mM D-lactate and 1 mM IPTG was monitored as OD_600nm(open symbols). The concentration of D-lactate in the supernatant was measured by HPLC (closed symbols). Averages and experimental errors from at least three independent growth experiments are shown.