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Growth of C. efficiens DSM44547 carrying either the empty vector pVWEx1 (squares) or the vector pVWEx1-dld (circles) in CgXII mineral medium containing 100 mM D-lactate and 1 mM IPTG. A representative growth curve is shown. The growth was monitored as OD600nm (closed symbols); the concentration of D-lactate in the supernatant was measured by HPLC (open symbols).
