Table 1.
List of bacterial strains, plasmids and oligonucleotides
| strain, plasmid or oligonucleotide | relevant characteristics or sequence | source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F- thi-1 endA1 hsdR17(r- m-) supE44 ΔlacU169 (ϕ80lacZΔM15) recA1 gyrA96 relA1 | [32] |
| Corynebacterium strains | ||
|
C. glutamicum ATCC 130302 |
ATCC [61] | |
| ::dld | dld inactivation mutant of ATCC 13032 | This work |
|
C. efficiens DSM44547 |
DSM | |
| Plasmids | ||
| pEKEx3 | SpecR; Ptac, lacIq | [24] |
| pEKEx3-dld | pEKEx3 containing dld from C. glutamicum and an artificial ribosome binding site | this work |
| pVWEx1 | KanR; Ptac, lacIq | [34] |
| pVWEx1-dld | pEKEx3 containing dld from C. glutamicum and an artificial ribosome binding site | This work |
| pK18mob | KanR; integration vector for C. glutamicum | [62] |
| pK18mob-dld | pK18mob carrying an internal fragment of dld | This work |
| Oligonucleotides | sequence |
underlined nucleotide (position in NC003450) |
| rbs-ndld | GTCGACAAGGAGATATAGATATGACGCAACCAGGACAGAC SalI | 955683 |
| cdld | GGAGCTCTTAGGCCCAGTCCTTGTGC SacI | 957398 |
| Ex-dld-fw | GCCCTGCAGGAAGGAGATATAGATATGACGCAACCAGGACAGAC SbfI | 955683 |
| Ex-dld-bw | GCGGTACCTTAGGCCCAGTCCTTGTGC KpnI | 957398 |
| dld1 | AATCATATGAGACGCAACCAGGACAGACCACC NdeI | 955683 |
| dld2 | AATGGATCCGGCCCAGTCCTTGTGCGGCGACGTGC BamHI | 957398 |
| Cg-dld-SalI-N498 | AAGTCGACAGCCAGATTCCAGATTCGCAAGGGT SalI | 956907 |
| Cg-dld-C1716-SalI | GGTCGACTTAGGCCCAGTCCTTGTGC SalI | 955683 |