Table 1.

S. Typhimurium proteins regulated by ppGpp

spot no.	STM no.	Gene	Fold	Anova (p)	Average fold change determined by qRT-PCR
Proteins expressed lower in ΔrelAΔspoT strain
002, 091	STM2884	sipC	0.1	0.006	NDa
005	STM0781	modA	0.3	0.032	0.67 ± 0.22
012	STM3169	Stm3169	0.3	0.004	0.18 ± 0.01c
014, 213	STM1796	treA	0.7	0.002	ECb
015	STM4403	cpdB	0.6	0.011	0.25 ± 0.06c
027	STM1954	fliY	0.5	0.033	ND
028	STM2884	sipC	0.1	0.009	ND
029	STM3557	ugpB	0.4	0.019	EC
029-2	STM0748	tolB	0.4	0.019	0.25 ± 0.03c
037	STM0209	htrA	0.6	0.032	0.60 ± 0.35
040	STM2638	rseB	0.3	0.011	0.88 ± 0.35
040-2	STM1478	ydgH	0.3	0.011	0.17 ± 0.06c
041	STM1375	ynhG	0.3	0.011	EC
056	STM1746	oppA	0.6	0.001	0.15 ± 0.05c
058	STM1746	oppA	0.5	0.006	0.15 ± 0.05c
059	STM1849	yliB	0.4	0.027	EC
060	STM3557	ugpB	0.3	0.006	EC
062	STM1091	sopB	0.2	0.036	ND
064	STM4319	phoN	0.1	0.014	0.54 ± 0.22
108	STM0435	yajQ	0.5	0.038	0.12 ± 0.05c
108-2	STM1440	sodC1	0.5	0.038	ND
153	STM3318	yhbN	0.6	0.047	0.28 ± 0.12c
154	STM4405	ytfJ	0.2	0.049	0.30 ± 0.02c
184	STM3348	degQ	0.4	0.038	EC
194	STM1720	yciO	0.3	0.028	14.22 ± 2.22c
Proteins expressed higher in ΔrelAΔspoT strain
004	STM3359	mdh	2.0	0.021	ND
006	STM3069	pgk	1.4	0.037	ND
008	STM2681	grpE	1.5	0.018	ND
068	STM3342	sspA	1.7	0.014	EC
081	STM2952	eno	1.7	0.014	ND
096	STM1700	fabI	1.8	0.041	ND
098	STM0232	accA	2.2	0.017	ND
101	STM3446	fusA	3.7	0.022	ND
109	STM4055	sodA	2.0	0.044	EC
115	STM3415	rpoA	1.5	0.043	EC
116	STM4184	aceA	1.6	0.007	ND
118	STM0737	sucB	1.7	0.006	ND
119	STM2660	clpB	3.7	0.035	ND
135	STM0735	sdhB	2.1	0.002	ND
142	STM3063	rpiA	1.8	0.022	ND
145	STM4190	pepE	1.5	0.003	ND
155	STM0734	sdhA	2.9	0.039	ND
186	STM3282	pnp	3.2	0.013	ND
187	STM3446	fusA	2.3	0.031	ND
210	STM1305	astD	1.8	0.007	EC
222	STM3502	ompR	1.7	0.025	ND
227	STM2378	fabB	1.6	0.035	ND
231	STM1746	oppA	1.8	0.012	ND

^aND, not determined.

^bEC, already identified as a ppGpp-regulated protein in E. coli by Traxler et al. [30].

^cmRNA level was significantly different between wild type and the ΔrelAΔspoT mutant.