Table 3.
Primers
| Name | Nucleotide sequence (5' to 3')a |
|---|---|
| Construction of the deletion mutants | |
| relA-FW | CGCCATCCCGCAATGGTTTACATAA |
| relA-RV | TCATTGTTCTGGCCATAACAGC |
| spoT-FW | CTTGAAAACCATCATTCGCGCTGAACG |
| spoT-RV | TCTGCGGTACGAATGATTGCAGAAACG |
| stm3169-red-FW | ACGTTCATTCACAACATCAGCGGTATTACTGGCCGGCTGTGTGTAGGCTGGAGCTGCTTC |
| stm3169-red-RW | ACATATTCTCGATGTATTCCAGATCCTTCGCTGACTGAGCCATATGAATATCCTCCTTAG |
| sseF-red-FW | AACAGAACGAAATATGAAAATTCATATTCCGTCAGCGGCAGTGTAGGCTGGAGCTGCTTC |
| sseF-red-RW | TGTCCATTAATGCAGGTGTAGTAGCAGATTGACAGAGCGCCATATGAATATCCTCCTTAG |
| pAC-tet-FW | TTGGTAGCTCAGAGAACCTTCGAAAAACCG |
| pAC-tet-RV | TCGCTCGCGTATCGGTGATTCATTCGCTA |
| Construction of plasmids for the complementations | |
| relA-FW2 | AGGCTCGAGGTCGCGGTAAGAAGT |
| relA-RV2 | ACAAGCTTACTGTCTGGGGTTTAC |
| ssrB-FW | GGGCTCGAGGAATATAAGATCTTATTAGTA |
| ssrB-RV | CCCGGATCCATACTCTATTAACCTCATTCT |
| stm3169-FW | CCGCTCGAGAACACACGTTCATTCACAACATCAG |
| stm3169-RV | GGAAGATCTATTCTCGATGTATTCCAGATCCTTC |
| Construcion of the lacZ fusions | |
| ssaG-Pro-FW | AAAGTCGACCAAATGCTCAGGTAGGAGGGC |
| ssaG-Pro-RV | AAAGGATCCATCATCGATTCTGGGTTGAGC |
| stm3169-Pro-FW | ACGCGTCGACGACGATTTAGCCGGTATGAAAATCA |
| stm3169-Pro-RV | CGGGATCCTTACATATTCTCGATGTATTCCAGA |
| Comfirmation of gene expression by qRT-PCR | |
| gyrA-FW | AAGAGCTCCTATCTGGATTATGC |
| gyrA-RV | TATTTACCGATTACGTCACCAAC |
| relA-FW | ATTGTGCCATTCACCTATCAGTT |
| relA-RV | GATATTTTTGTCACGATCCTGCT |
| invF-FW | ATCGCTGCTGAATAGTGTAGAAG |
| invF-RV | CATTTGTCTGCCAATTGAATAAT |
| stm0209-FW | CCTGAACGTAGAAAATTACGAGA |
| stm0209-RV | GTCAGGTTTTTCACCATGTTACT |
| stm0435-FW | GTCAATCAGTTGCTCGATATTCTG |
| stm0435-RV | TTTAATCAGCTTGACGATTTTCTTC |
| stm0748-FW | TGAACCTGTACGTTATGGATCTC |
| stm0748-RV | CGCCGTTAATGTTCATTTTATAC |
| stm0781-FW | GAAGGCAAGATCACCGTATTT |
| stm0781-RV | CTGATCAGCAGAGATGAAGAGAT |
| stm1478-FW | ACAAAAGTTGAGGAGCTGAATAAAG |
| stm1478-RV | GCCACTGACGCGTAATATGATAA |
| stm1720-FW | TTGGTTGTAAAATTGAAGACAAAGG |
| stm1720-RV | GTCCCCTTCAGGATAAAGGTGTAAT |
| stm1746-FW | CGAATTATTCCAGAAACTGAAGAAA |
| stm1746-RV | ATCGCCCTGATTTTTAACCTTATTA |
| stm2638-FW | TATTCTGACGGTCTGTTTAGCTTTT |
| stm2638-RV | GTACTGCCCTGAATTTGATACTGTC |
| stm3169-FW | GTTACCAGAATAATGTCGCAGCTAT |
| stm3169-RV | AATCATCCACATAAAAAGAATCTGG |
| stm3318-FW | CAAACTCAGCCTTAATCTTATGC |
| stm3318-RV | ACTTTATCGGCGTTGATCTTAAT |
| stm4319-FW | ATTAGTATTATCCGAGGCCAGAC |
| stm4319-RV | CAGTCTTGCAAACTCTACTGCTC |
| stm4403-FW | ATTGATATTCACAGCAACAAACC |
| stm4403-RV | AGGTCAGGTTTTTAATACGTTCC |
| stm4405-FW | CGAACTGACGTTGAATAAAGATGAG |
| stm4405-RV | AATTGTGGTCGTCTGGTATCTGT |
aUnderlined part indicates P1 or P2 site for pKD4, or restriction sites.