Table 3.

Primers used in the present study

Name	Sequence	References	Target gene (amplicon size)	Annealing temperature (°C)
lfb1-F	CATTCATGTTTCGAATCATTTCAAA	[15]	lfb1 (331 bp)	60
lfb1-R	GGCCCAAGTTCCTTCTAAAAG
secY-F	ATGCCGATCATTTTTGCTTC	[18]	secY (549 bp)	60
secY-R	AGTTGAGCCCGCAGTTTTC
SecYIVF	GCGATTCAGTTTAATCCTGC	[9]	secY (202 bp)	54
SecYIV	GAGTTAGAGCTCAAATCTAAG
pfkB-F	CCGAAGATAAGGGGCATACC	[20]	pfkB (559 bp)	52
pfkB-R	CAAGCTAAAACCGTGAGTGATT
pntA-F	TGCCGATCCTACAACATTA		pntA (637 bp)	52
pntA-R	AAGAAGCAAGATCCACAACTAC
sucA-F	AGAAGAGGCCGGTTATCATCAG		sucA (559 bp)	52
sucA-R	CTTCCGGGTCGTCTCCATTTA
tpiA-F	AAGCCGTTTTCCTAGCACATTC		tpiA (554 bp)	52
tpiA-R	AGGCGCCTACAAAAAGACCAGA
mreA-F	GTAAAAGCGGCCAACCTAACAC		mreA (601 bp)	45
mreA-R	ACGATCCCAGACGCAAGTAA
glmU-F	GGAAGGGCACCCGTATGAA		glmU (556 bp)	50
glmU-R	TCCCTGAGCGTTTTGATTT
fadD-F	AGTATGCGTATCTTCCTCCTT		fadD (576 bp)	50
fadD-R	TTCCCACTGTAATTTCTCCTAA