Table 1. Bacillus subtilis strains used in this study.
Strain | Relevant genotype or descriptiona | Origin or constructionb |
168 | trpC2 | Bacillus Genetic Stock Center |
ATCC 6633 | American Type Culture Collection | |
ATCC 9372 | American Type Culture Collection | |
ATCC 6051 | American Type Culture Collection | |
PG01 | CTSCCV | |
NDmedical | Non-domesticated, isolated from endoscope washer-disinfectors [35] | |
NDfood | Non-domesticated, isolated from dairy product (ISHA) | |
BSB168 | trp+ derivative of 168 | [31] |
GM2812 | amyE::Phyp-gfp (spec) | pDR146 (D. Rudner)→BSB168 |
GM2815 | amyE::Phyp-gfp (spec), ΔdegQ::cat | BG4138 [51]→GM2812 |
GM2817 | amyE::Phyp-gfp (spec), degU::neo | GM719 [52]→GM2812 |
GM2821 | amyE::Phyp-gfp (spec), degU32-kan | QB4371 [53]→GM2812 |
GM2828 | amyE::Phyp-gfp (spec), yvfV::pMUTIN2 (ery) | BSFA1123→GM2812 |
GM2830 | amyE::Phyp-gfp (spec), yvfW::pMUTIN2 (ery) | BSFA1124→GM2812 |
GM2832 | amyE::Phyp-gfp (spec), gtaC::pBS640 (cat) | L16601gtaC [54]→GM2812 |
GM2812ylbF1 | amyE::Phyp-gfp (spec), ylbF::pMUTIN4 (ery) | BSFA3233 [40]→GM2812 |
GM2812ymcA1 | amyE::Phyp-gfp (spec), ymcA::pMUTIN4 (ery) | BSFA2603 [40]→GM2812 |
GM2850 | amyE::Phyp-gfp (spec), yxaB::pKN3 (ery) | MM1701 [44]→GM2812 |
GM2851 | amyE::Phyp-gfp (spec), ΔabrB::cat | MM1717 [44]→GM2812 |
GM2853 | amyE::Phyp-gfp (spec), ΔabrB::cat, yxaB::pKN3 (ery) | MM1707 [44] àGM2812 |
GM2855 | amyE::Phyp-gfp (spec), sigX::pMUTIN2 (ery) | BSFA94→GM2812 |
GM2857 | amyE::Phyp-gfp (spec), yqxM::pMUTIN3 (ery) | BSFA4767 [40]→GM2812 |
GM2888 | amyE::Phyp-gfp (spec), Δhag::cat | OMG954 [55]→GM2812 |
spec, cat, neo, kan and ery stand for spectinomycin, chloramphenicol, neomycin, kanamycin and erythromycin resistance markers, respectively.
CTSCCV, centre technique de la salaison, de la charcuterie et des conserves de viandes; ISHA, Institut Scientifique d'Hygiène et d'Analyse; BSFA strains were constructed during the “Bacillus subtilis functional analysis programme” [56].