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. 2011 Jan 18;6(1):e16177. doi: 10.1371/journal.pone.0016177

Table 1. Bacillus subtilis strains used in this study.

Strain Relevant genotype or descriptiona Origin or constructionb
168 trpC2 Bacillus Genetic Stock Center
ATCC 6633 American Type Culture Collection
ATCC 9372 American Type Culture Collection
ATCC 6051 American Type Culture Collection
PG01 CTSCCV
NDmedical Non-domesticated, isolated from endoscope washer-disinfectors [35]
NDfood Non-domesticated, isolated from dairy product (ISHA)
BSB168 trp+ derivative of 168 [31]
GM2812 amyE::Phyp-gfp (spec) pDR146 (D. Rudner)→BSB168
GM2815 amyE::Phyp-gfp (spec), ΔdegQ::cat BG4138 [51]→GM2812
GM2817 amyE::Phyp-gfp (spec), degU::neo GM719 [52]→GM2812
GM2821 amyE::Phyp-gfp (spec), degU32-kan QB4371 [53]→GM2812
GM2828 amyE::Phyp-gfp (spec), yvfV::pMUTIN2 (ery) BSFA1123→GM2812
GM2830 amyE::Phyp-gfp (spec), yvfW::pMUTIN2 (ery) BSFA1124→GM2812
GM2832 amyE::Phyp-gfp (spec), gtaC::pBS640 (cat) L16601gtaC [54]→GM2812
GM2812ylbF1 amyE::Phyp-gfp (spec), ylbF::pMUTIN4 (ery) BSFA3233 [40]→GM2812
GM2812ymcA1 amyE::Phyp-gfp (spec), ymcA::pMUTIN4 (ery) BSFA2603 [40]→GM2812
GM2850 amyE::Phyp-gfp (spec), yxaB::pKN3 (ery) MM1701 [44]→GM2812
GM2851 amyE::Phyp-gfp (spec), ΔabrB::cat MM1717 [44]→GM2812
GM2853 amyE::Phyp-gfp (spec), ΔabrB::cat, yxaB::pKN3 (ery) MM1707 [44] àGM2812
GM2855 amyE::Phyp-gfp (spec), sigX::pMUTIN2 (ery) BSFA94→GM2812
GM2857 amyE::Phyp-gfp (spec), yqxM::pMUTIN3 (ery) BSFA4767 [40]→GM2812
GM2888 amyE::Phyp-gfp (spec), Δhag::cat OMG954 [55]→GM2812
a

spec, cat, neo, kan and ery stand for spectinomycin, chloramphenicol, neomycin, kanamycin and erythromycin resistance markers, respectively.

b

CTSCCV, centre technique de la salaison, de la charcuterie et des conserves de viandes; ISHA, Institut Scientifique d'Hygiène et d'Analyse; BSFA strains were constructed during the “Bacillus subtilis functional analysis programme” [56].