Figure 1. Identification of ADNP as an H3K9me3 associated factor.

(A) The indicated histone H3 peptides were used in pulldown experiments of HeLa S3 cell nuclear extract. Beads without coupled peptides were used as control (mock). Specifically recovered proteins were run on SDS-PAGE and stained with Coomassie Blue. Proteins were identified by MS/MS analysis. The positions of ADNP, HP1 and CDYL1 proteins are indicated on the right. Molecular weight (MW) markers are indicated on the left. (B) Amino acid sequence of ADNP. The highlighted (grey) peptides were identified by MS/MS analysis. (C) Western blot analysis of a histone H3 peptide pulldown experiment of HeLa S3 cell nuclear extract as in (A) using an anti-ADNP antibody.