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. 2001 Feb 27;98(5):2849–2854. doi: 10.1073/pnas.051628598

Figure 4.

Figure 4

5α-reductase Type I mRNA (A), 5α-reductase Type I protein (B), and 3α-HSOR (C) mRNA content in frontal cortex of group-housed (GH, open bars) and socially isolated (SI, filled bars) mice. Each value is the mean ± SE of at least six animals. *, P < 0.01 with Student's t test comparison. mRNAs were quantified by competitive RT-PCR with mutant internal standards (16). (Inset in B) Relative content of 5α-reductase Type I protein (5α-R, molecular mass ≈30 kDa) was estimated by Western blot using OD ratio with β-actin (47 kDa) as a control for overall protein content and blotting efficiency.