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Dual-luciferase reporter assay to measure TF activity. MCF-7:WS8 cells were transfected in parallel with reporter constructs containing enhancer elements for specific TFs (AP1, ER, NFκB, SP1, E2F1, and GATA3) and TA vector control in combination with a Renilla luciferase reporter to normalize for transfection. Cells were lysed 24 h after transfection and TF activitywas quantified using a dual-luciferase assay. Values are means ± SD of three independent experiments done in triplicate.
