Table 1.
Quantification of polysomal loading of Ldhc and Smcp mRNAs at different stages by different methods
| mRNA(Age)1 | Nycodenz2 Phos3 | Nycodenz qPCR3 | Sucrose2 Phos | Sucrose qPCR | Immuno2 Phos |
|---|---|---|---|---|---|
| LdhC (21 dpp) | 33.4, 36.04 | 34.6 ± 3.2 (6) | 57.7, 58.1 | 51.0 ± 9.3 (6) | 5ND |
| LdhC (Adult) | 32.6 ± 2.7 (3) | 28.4 | 56.3 ± 2.1 (9) | 56.8 ± 2.7 (5) | ND |
| Smcp (21 dpp) | 2.2 ± 0.6 (3) | 3.0, 4.3 | 6.7, 8.3 | 3.7 ± 2.8 (4) | ND |
| Smcp (Adult) | 32.8 ± 3.2 (3) | 33.8 | 35.3 ± 4.9 (11) | 29.8 ± 9.3 (4) | ND |
| Prm1 (25 dpp) | ND | ND | ND | ND | 9.8 ± 1.9 (3) |
| Prm1 (32 dpp) | ND | ND | ND | ND | 29.8 ± 3.7 (3) |
| Prm1 (Adult) | ND | ND | 24.5 ± 6.5 (3) | ND | ND |
1Name of species of mRNA and (age of testes analyzed)
2Method of separation of free-mRNPs and polysomal mRNAs: sucrose gradient, Nycodenz gradient, immunoprecipitation of HA-epitope tagged RP22 [16]
3Method of quantification of levels of mRNA: phosphorimage of Northern blots or slot blots or RT-qPCR
4Percent of mRNA associated with polysomes. The results are expressed and mean and standard deviation with number of replicate determinations in parentheses. The absolute values are given when there one or two replicates. The values are derived from references [4-6,16].
5ND, not determined