Figure 3. Microarray analysis of the transcriptome response to pulsatile versus continuous glucocorticoid treatment.

(A) Principal components analysis (PCA) of microarray data to establish relationships between the samples using all genes in the microarray dataset. Control, pulsatile cortisol delivery (PULSE), continuous 100 ng/ml (100), and continuous 200 ng/ml (200) are indicated on the plots. There is a clear separation between all the cortisol treatments and control in the first component. (B) When cortisol treatments are considered separately pulsatile treatment segregates from the two continuous treatments in the third component.