Figure 6. Expression of total GR and Ser 211 phosphorylated GR (P-GR) in HeLa cells after different flow-through schedules.
Cells were set up and treated with control (C), pulse (P), c100 (C1), & c200 (C2) treatment schedules, as for the microarray experiments. Whole cell extracts were prepared and immunoblotting was performed using the indicated antibody.