Table 1.
Quantitative reverse transcriptase-PCR primers used for detecting expressions from wheat polyamine biosynthesis genes.
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') | Locus | GenBank accession |
|---|---|---|---|---|
| Ornithine decarboxylase (ODC) | AGCGTTACTTCGGGGAGCTT | ATTGTGAAGGCGGTCTCG | Os09g37120 | HM770451 |
| Arginine decarboxylase 1 (ADC1) | CACCAAGATACCAGGCCACT | GTGGAAGTGCAGCAACTTGA | Os04g01690 | HM770446 |
| Arginine decarboxylase 2 (ADC2) | AGGAGGAGGAGCTCGACATT | GCCGAACTTGCCCTTCTC | Os06g04070 | HM770449 |
| Agmatinase/Arginase | GGGAAGAGATTTGGTGTGGA | TCACACCTTCCCCAAGTTTC | Os04g01590 | HM770450 |
| S-adenosylmethionine decarboxylase 2 | GCGTCCTCATCTACCAGAGC | CTTGCCTTCCTTGACCAGAG | Os04g42090 | HM770448 |
| Spermidine synthase 1 | TGATTCAGGACATGCTTTCG | CCCAATTGCACCACTAGGAT | Os02g15550 | HM770442 |
| Spermidine synthase 2 | GCAAAAATCCAATGACACCA | TGTGGCTGCACACACATCTA | Os06g33710 | HM770452 |
| Argininosuccinate synthase | ACGCTGAAGGTTTCATCAGG | TAGATGCCCTTCTCCAGCAT | Os12g13320 | HM770445 |
| Argininosuccinate lyase | GTTGAACAGTTGGAGCGTGA | GAGTCCAGTTCCAGCCAAAG | Os03g19280 | HM770447 |
| Carbamoyl-phosphate synthase | TTGGAAAATTGTTGGTGTTG | CCCATTCATATGGAGCATC | Os02g47850 | HM770444 |
| Ornithine carbamoyl transferase | ATGAAGCCCTGATGGAGATG | ATGGCACCGTCTGTTACCTC | Os02g47590 | HM770443 |
| 18 S rRNA* | CGACCTACTCGACCCTTCGGCCGG | CGATGCCGGAAACACGACCCGG | - | |
| Peroxidase† (TaPERO) | GAGATTCCACAGATGCAAACGAG | GGAGGCCCTTGTTTCTGAATG | - | X56011 |
| 18 S rRNA fungal* | GTCCGGCCGGGCCTTTCC | AAGTCCTGTTTCCCCGCCACGC | - | |
| TRI5* | CACTTTGCTCAGCCTCGCC | CGATTGTTTGGAGGGAAGCC | FGSG_03537 | |