Figure 6.

Globaltest analysis results. Example covariate plot for the miRNA-125b-5p transcript (A) displaying the individual contributions of its 203 isomirs to the overall test statistics for differential expression. Significantly contributing transcripts are indicated by the dark line in the hierarchical tree. B: Zoomed section of the hierarchical tree depicting the subset of 23 significantly contributing isomir sequences. Positions and the detected nucleotide of variations are indicated, e.g., 17T# indicates that nucleotide 17 in the reference sequence was a 'T'. C: Isomir expression plots between the Heart and Embryo showing uniform differential isomir expression patterns for mir-499-3p, mir-125b-5p, mir-20b-5p and mir-219-3p, respectively.