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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2010 May 4;6(1):130–141. doi: 10.1007/s11481-010-9218-7

Fig. 4.

Fig. 4

Effects of chronic morphine exposure on IL-4-induced MHC-II expression in B lymphocytes. Sprague-Dawley rats (n=8) were administered either saline or morphine twice daily using the dosing schedule as described in “Materials and methods”. Acutely treated animals received morphine (10 mg/kg, s.c.) on the day of the experiment. Two hours after the final injection, peripheral blood mononuclear cells were isolated and cultured with rrIL-4 (0.1– 1,000 ng/ml). Stained cells were fixed using 1% paraformaldehyde prior to analysis using a FACSort flow cytometer. Data are expressed as MFI ± SEM. Data repeated in a subsequent experiment. *p<0.05 compared to all other treatment groups (ANOVA, Newman–Keuls)