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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Clin Cancer Res. 2010 Nov 8;17(2):267–274. doi: 10.1158/1078-0432.CCR-10-1937

Figure 4. Cytoplasmic localization of galectin-3 in CD18/HPAF-derived cells, and the effect of asialofetuin on galectin-3-mediated adhesion of CD18/HPAF cells to HUVECs.

Figure 4

A, Immunoblot analysis detected galectin-3 expression in CD18/HPAF-derived cells lysates, but not in the enriched cell culture media. Here β-actin and nidogen were taken as internal controls. B, Immunofluorescence analysis showed the cytoplasmic localization of galectin-3 in CD18/HPAF-derived cells. C, Preincubation of CD18/HPAF cells with asialfetuin at a concentration of 1mg/mL abolished the galectin-3 mediated enhanced adhesion of CD18/HPAF cells to HUVECs.