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. Author manuscript; available in PMC: 2011 Jan 19.
Published in final edited form as: Proteins. 2008 May 15;71(3):1360–1372. doi: 10.1002/prot.21824

Table I.

Summary of Mutation of Key Polymerase Residues as Inferred from the Interactions Seen for the G:C Simulations, and the Effects on a G:A Mismatch and Oxidatively Damaged G at the Insertion Site On These Interactions

Residue Effect of mutationa Residue-DNA interactionb
R615

  • 23-Fold decrease in DNA binding

  • 2-Fold decrease in fidelity

  • 300-fold decrease in catalytic activity

  • Contacts dNTP sugar (O4′) and A(Pn−1) base (N3)
R714

  • Decrease in replication fidelity

  • Contacts G(Tn) base (N2 hydrogen)
Q797

  • 40-Fold decrease in DNA binding

  • 3–10 Fold increase in fidelity

  • Contacts T(Tn−1) base (O2) and C(Tn−2) sugar (O4′)
H829

  • Increase in replication fidelity

  • Decrease in DNA binding

  • Contacts – A(Pn−1) sugar (O4) and G (Pn−2) base (N3)
a

From Refs. 76 and 78.

b

Subscripts n − 1, n − 2 represent bases further down in the DNA duplex.