Fig. 9.

Changes in the different layers in the different lobules over time, as shown by Stevenel’s staining. a-a”’ All the layers of the cerebellum were distinct and well organized in control mice, as shown in lobule IX in which the thickness (blue arrow) of the external granular layer (EGL), and numbers of GC were reduced linearly with progression of age (ML molecular layer, PCL Purkinje cell layer, IGL internal granular layer). b-b”’ In lobule X of mutant cerebellum, all the layers were organized normally at all ages. c-c”’ In central lobules shown in lobule VII of Neurod1 mutant cerebellum, the EGL was expanded (blue arrow), and the number of GCs were abnormally increased in P11 (c’) from P7 (c), although by P15, GCs were dispersed and scattered and many pyknotic nuclei (red arrows) and mitotic nuclei (green arrowheads) were observed in lobule VII, with no ML and no definite PCL (P). d-d”’ In the anterior lobules shown in lobule V of Neurod1 mutant mice, the layers were reasonably well-organized, but pyknotic nuclei and mitotic nuclei were observed in EGL in P7 and P11 mice (d, d’). Bars 10 μm