TABLE 2. Results of adjudin toxicity study.

Results of standard toxicity studies on adjudin performed by licensed toxicologists according to FDA guidelines. [Adapted and reproduced from Mruk DD, Wong CH, Silvestrini B, and Cheng CY (2006) A male contraceptive targeting germ cell adhesion. Nat Med 12:1323–1328. Copyright © 2006 Nature Publishing Group. Used with permission.]

Test	Regimen and Dosing	Administration Route	Species	Results
Acute toxicity study	100 mg/kg b.wt. (1 dose)	Intraperitoneal	Mouse; males (n = 3)	Negative
	1000 mg/kg b.wt. (1 dose)	Intraperitoneal	Mouse; males (n = 3)	Negative
Acute toxicity study	2000 mg/kg b.wt. (1 dose)	Oral	Rat; males (n = 5) and females (n = 5)	Negative
Bacterial mutation assay	0.586–18.8 µg/plate	In vitro	Salmonella typhimurium andEscherichia coli	Negative in both species
Mammalian erythrocyte micronucleus test	500, 1000, and 2000 mg/kg b.wt. (1 dose)	Oral	Mouse; males (n = 5) and females (n = 5)	Negative in all three dosings
Mammalian chromosome aberration test	6.25–100 µg/ml	In vitro	CHO cells	Negative in inducing numerical chromosome aberrations; positive in inducing structural chromosome aberrations
29-Day subchronic toxicity study	50 mg/kg b.wt. every day for 29 days	Oral	Rat; male (n = 10) and female (n = 10)	Negative in female; positive in male