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. 2011 Jan 17;208(1):167–180. doi: 10.1084/jem.20101850

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© 2011 Hufford et al.

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Figure 6. — CD8⁺ T cell effector activities upon co-culture with influenza-infected hematopoietic and nonhematopoietic cells. (A and B) Lung suspensions of infected BALB/c mice (8 dpi) were collected and pooled. CD8⁺ cells were positively selected via MACS bead separation. CD8⁺ cells (effectors) were co-cultured with influenza-infected cells (targets) in indicated effector/target ratios ± α-CD107a/b (FITC). CD8⁺ T cell degranulation (CD107a/b⁺; A) and IFN-γ–producing CD8⁺ T cells after co-culture (B) are shown. Percentage max is the proportion of effector activity with respect to maximum production of effector activity within one experiment. Four independent experiments (two to three pooled mice/experiment) as mean ± SEM are shown. (C) Lung suspensions of infected BALB/c mice (8 dpi) were co-cultured in a 1:1 ratio with influenza-infected BMDCs for 6 h in the presence of isotype or blocking CD80 and CD86 antibodies. Representative flow profiles (right) and percentage (left) of IFN-γ⁺CD8⁺ T cells after in vitro restimulation are shown. Two independent experiments (n = 5) as mean ± SEM are shown. A paired t test was used to test statistical significance. (A–C) Considered a significant difference at *, P < 0.05.