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. 2011 Jan 17;208(1):81–90. doi: 10.1084/jem.20101574

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© 2011 Sabatino et al.

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Figure 3. — Dominance of proinflammatory low affinity myelin-reactive CD4⁺ T cells during EAE. (A and B) Representative tetramer (A) and 2D binding (B) of CNS-infiltrating CD4⁺ T cells to MOG_38–49-IA^b and hCLIP_103–117-IA^b. (C) The mean frequency ± SEM of MOG_35–55-specific binding by tetramer and 2D analysis was based on three experiments performed in parallel, and CNS tissue was pooled together from 6–10 mice per experiment (*, P = 0.004). (D) Representative frequency of cytokine-producing (IFN-γ and TNF) CD4⁺ T cells isolated from the CNS during acute EAE after stimulation with MOG_35–55 or no peptide (no stimulation [stim]). (E) The mean percentage ± SEM of CNS-infiltrating MOG_35–55 CD4⁺ T cells was compared in parallel by MOG_38–49-IA^b tetramer and the percentage producing cytokine (IFN-γ and TNF) upon stimulation with MOG_35–55 (no stimulation background subtracted) from two independent experiments (CNS tissue pooled from 12–24 mice; *, P = 0.04).