Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 17;208(1):3–11. doi: 10.1084/jem.20100027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Viret et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — The reduced response of Tssp^−/− mice to HEL is a CD4 T cell property. (A) IL-2 production by the HEL-specific T cell transfectant BW-HEL upon stimulation with splenic APCs from Tssp^−/− (KO) or WT control mice loaded with graded concentrations of HEL_12–27 peptide or HEL protein, as indicated. One representative experiment out of four performed is shown. (B) WT or Tssp^−/− (KO) mice were primed with HEL protein (left) or HEL_12–27 peptide (right). 11 d later, CD4 T cells were isolated from the draining LN and restimulated in vitro with WT spleen APCs and graded concentrations of HEL_12–27 peptide. (C) WT and Tssp^−/− mice were primed in vivo, and their CD4 T cells were restimulated in vitro with WT spleen APCs and either HEL protein or HEL_12–27 peptide, as indicated. Results are expressed as the ratio of the proliferation of TSSP-deficient T cells divided by that of WT control. The figure compiles the results of three to five independent experiments. Each symbol corresponds to one mouse. Horizontal bars represent the mean. One-sample t test showed that the responses of WT and Tssp^−/− mice are significantly different for all stimulation conditions (P < 0.002). (D) WT and Tssp^−/− (KO) mice were primed in vivo with either WT or TSSP-deficient DCs pulsed with 40 ng HEL_12–27 peptide, and their CD4 T cells were restimulated in vitro with WT spleen APCs and a titrated amount of HEL_12–27 peptide. One representative experiment is presented in the left panel. The right panel combines results of three independent experiments expressed as the ratio of the proliferation of WT or TSSP-deficient T cells (X) divided by the mean value of the WT controls in the same experiment. Each symbol corresponds to one mouse. Horizontal bars represent the mean. Significant p-values are shown. (E) Purified LN CD4 T cells from HEL- or OVA-immunized WT and Tssp^−/− mice were labeled with CFSE and stimulated with WT splenocytes together with HEL_12–27 peptide, OVA protein, or anti-CD3/CD28 antibodies, as indicated. Cell division was evaluated by analyzing CFSE dilution on gated CD4 T cells at day 3 and 6 of activation. The percentage of divided WT or TSSP-deficient (KO) CD4 T cells is shown. One representative experiment out of four performed is shown.