Table I.

Vβ6-Cβ joint of HEL_12–27-specific CD4 T cells

Sequence no.	Sequences (aa)	Jβ	L	nCh	WT				Tssp−/−
					1	2	3	4	1	2	3	4	5
1	SIWQGRV	1.1	7	1	1
2	SIGGNTEV	1.1	8	−1				1
3	WDRGGATEV	1.1	9	−1			1
4	SDRVHSDY	1.2	8	0		1
5	SIDTSGNTL	1.3	9	−1		1	1
6	SIRTGGFEQ	2.1	9	0	1
7	SRTDYAEQ	2.1	8	−1		1
8	SIWGGNYAEQ	2.1	10	−1		1
9	SSRDRNYAEQ	2.1	10	0		1
10	SIRDRNYAEQ	2.1	10	0			1
11	SVRDKNTGQL	2.2	10	1		1
12	SIWGSAETL	2.3	9	−1		1	1
13	SRLAHRAETL	2.3	10	2		1
14	SLGTAETL	2.3	8	−1			1
15	TPAGSSAETL	2.3	10	−1			1
16	SIVGGRNTL	2.4	9	1		1
17	SGTQDTQ	2.5	7	−1			1
18	SIDWGNQDTQ	2.5	10	−2				1
19	SIGGTGGYEQ	2.7	10	−1				1
20	SIAWTGGYEQ	2.7	10	−1				1
21	SIEDRYEQ	2.7	8	−2	1
22	SIADRYEQ	2.7	8	−1	2
23	SINRGEQ	2.7	7	0		1
24	SINERL	1.4	6	0	1							2
25	SIGRGERL	1.4	8	1	1								1
26	SIGTISNERL	1.4	10	0		1							1
27	SISNYAEQ	2.1	8	−1		1	2	2	1
28	RGQGNNYAEQ	2.1	10	0		1						1
29	TPDRNTGQL	2.2	9	0		1	2	1					2
30	SIISAETL	2.3	8	−1			1		1			2
31	SLESAETL	2.3	8	−2		1	1	2	1				2
32	IFDRGSAETL	2.3	10	−1	3				8	7			7
33	SILWGPYEQ	2.7	9	−1	1								3
34	SSGLSYEQ	2.7	8	−1				2		1
35	SIYRGHGNTL	1.3	10	2							1
36	SIGFSNERL	1.4	9	0								1
37	TLTPGAEQ	2.1	8	−1						1
38	SILGGNYAEQ	2.1	10	−1					4	4	2
39	SIVWGGIDAEQ	2.1	11	−2								1
40	SPDWGRNYAEQ	2.1	11	−1							1
41	RQDTQ	2.2	4	0						1
42	SLLQNTGQL	2.2	9	0							1
43	SLDRNTGQL	2.2	9	0								1
44	SMGDITGQL	2.2	9	−1							2
45	SWDFLNTGQL	2.2	10	−1					2	4			2
46	SIRTGNTGQL	2.2	10	1								1
47	TGAGSSAETL	2.3	10	−1									1
48	WQTDQ	2.5	5	−1								1
49	SRLGGDTQ	2.5	8	0							1
50	SIGIEQ	2.7	6	−1							2
51	SIVGAGEQ	2.7	8	−1								1
52	SRGLGDYEQ	2.7	9	−1							1
53	SIRRGHYEQ	2.7	9	2					4	1
54	SMGWGGLSYEQ	2.7	11	−1								1

Compilation of 54 distinct CDR3 sequences of Vβ6 TCR chain from purified HEL-responsive CD4 T cells from four WT (1–4) and five Tssp^−/− (1–5) HEL-immunized mice. For each sequence, the relevant Jβ segment, CDR3 length (L), and CDR3 net charge (nCh) are indicated. Negatively and positively charged amino acids are shown underlined and in bold, respectively. The number of times each sequence occurred in individual mice is shown and appears when corresponding to dominant CDR3 sequences. The 54 individual sequences correspond to a total of 132 clones analyzed. .