Table II.

Vβ6-Jβ2.1 joint of HEL_12–27-specific CD4 T cells

Sequences (aa)	L	nCh	WT				Tssp−/−
			1	2	3	4	1	2	3	4	5
SRTDYAEQ	8	−1	2	4		1
SIRTGGFEQ	9	0	1
SRQGGDTQY	9	0		1
SIWGGNYAEQ	10	−1		1
SSRDRNYAEQ	10	0		1
SIRDRNYAEQ	10	0			1
FPRIPGKNYAEQ	12	1	1
SISNYAEQ	8	−1	1	1	8	8	1	1		1	1
SIWDRYAEQ	9	−1	2			2				1
STGGNYAEQ	9	−1		2							3
SILGGNYAEQ	10	−1	2		4	3	6	4	5	2	2
RGQGNNYAEQ	10	0		2			1	1		1
TLTPGAEQ	8	−1						1
RQQNYAEQ	8	0									1
SIWDGYAEQ	9	−2						1
PIQENYAEQ	9	−2						2
SILWGPYEQY	10	−1									1
RGQGNRCAEQ	10	1						1
SIVWGGIDAEQ	11	−2								1
SPDWGRNYAEQ	11	−1							2		4

Compilation of 18 distinct CDR3 sequences derived from 92 clones of Vβ6-Jβ2.1 joints from purified HEL-responsive CD4 T cells from four WT (1–4) and five Tssp^−/− (1–5) HEL-immunized mice. For each sequence, the CDR3 length (L) and CDR3 net charge (nCh) are indicated. Negatively and positively charged amino acids are shown underlined and in bold, respectively. The number of times each sequence occurred in individual mice is shown.