Differential expression of miR-27a and b in fast and slow muscles and in response to Dex treatment in vitro. A–C: expression of miR-27a (A), miR-27b (B), and U6 control RNA (C) in the TA and Sol muscle. Both miR-27a and b were expressed at a significantly higher level in Sol compared with TA. Bars represent means ± SE for n = 4 muscles each. *Significantly different from TA, P < 0.05. D–F: expression of miR-27a (D), miR-27b (E), and miR-499 (F) in GFP-cotransfected vehicle-treated and GR-cotransfected Dex-treated C2C12 myotubes. MiR-27a but not miR-27b was expressed at a significantly lower level in GR-cotransfected Dex-treated myotubes. Bars represent means ± SE for n = 3 different experiments. *Significantly different from GFP-cotransfected, vehicle-treated myotubes, P < 0.05.