Fig. 2.

Steady-state hSGLT2 Na⁺/d-glucose cotransport is electrogenic and membrane potential (V_m) and temperature dependent. A: whole cell recordings at 37°C and a V_m of −60 mV in HEK293T cells expressing hSGLT2 revealed inward current upon addition of glucose to the extracellular Na⁺ solution. Upon returning to Na⁺-only, current was restored to baseline. In Na⁺-free buffer (i.e., 150 mM cholineCl), there was no glucose-induced current (Fig. 3B). B: current-voltage (I-V) relationships of the glucose-induced current (□, 22°C; ■, 37°C).