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Labeling of PAR2 in proximal ileum. Isolated mouse proximal ileum from wild-type mice (left) and PAR2−/− mice (right) was fixed, sectioned, and stained with PAR2 antibody 9717 (green) and TOPRO3 (blue) for detection of cell nuclei, and phalloidin (red) for detection of cell cytoskeleton and microvilli. Bottom: higher magnifications of boxed regions at top. (Arrows indicate apical and basolateral PAR2.)
