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Steady-state IL-18 and IL-18BP mRNA levels in control and ischemic atrial tissue. Tissues obtain after conditions described in Fig. 2 were snap-frozen in liquid nitrogen and kept frozen at −70°C. After homogenization in Tri-Reagent and mRNA isolation, levels of IL-18 and IL-18BP mRNA were determined by reverse transcription-coupled PCR. Data are from one of two subjects evaluated. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
