Fig. 3.
IL-6, monocyte chemoattractant protein-1 (MCP-1), TNFα, IL-1α, and IL-1β mRNA were upregulated in visceral adipocytes isolated from obese Zucker rats. Real-time RT-PCR measurements of IL-6, MCP-1, TNFα, IL-1α, and IL-1β mRNA levels in adipocytes. The data were normalized to 18S rRNA. The fold changes were measured relative to lean control and calculated with the 2−ΔΔCT method. All data represent the mean ± SE from 3 independent experiments, and P values indicate that values are statistically significantly different (P < 0.001–0.05) compared with lean rats.