Table 1.

Primer sequences used in real-time RT-PCR amplification of cDNA from rat adipocytes/adipose tissue

Rat Genes	Forward Primers	Reverse Primers
Leukocyte type 12-LO	5′-GATGGG TGTCTACCG CATCC-3′	5′-CCTC TCCATGC TGTCCAACC-3′
5-LO	5′-GGCACCGACGAC TACATTTACC-3′	5′-ACCCAGT TCTTCATCCACAGTGAC-3′
5-FLAP	5′-TCAAGAG GC TG TG G GCAATG-3′	5′-CAGT TCTGGTTGGCAGTGTAGACC-3′
IL-6	5′-AC CAC T TCACAAGTCGGAGG-3′	5′-ACAGTGCATCATCGCTGT TC-3′
TNFα	5′-GGTGATCG GTCCCAACAAG GA-3′	5′-CACGCTGGC TCAGCCACTC-3′
MCP-1	5′-C CTG C TGC TACTCATTCAC-3′	5′-TCTCAC TTGG TTCTG GTC C-3′
18S	5′-CCCAGTAAGTGCGGGTCATA-3′	5′-GGCCTCACTAAACCATCCAA-3′
Cychlophilin	5′-AGCACTG GGGAGAAAGGATT-3′	5-CATGCCTTCTTTCACCTTCC-3′

12- and 5-LO, 12- and 5-lipoxygenase, respectively; 5-FLAP, 5-LO-activating protein; MCP-1, monocyte chemoattractant protein-1.