Fig. 7.

Phosphorylation of UBF1, Erk, and p70S6 kinase is increased in the renal cortex of rodents with type 1 and type 2 diabetes. Rats were administered with streptozotocin to induce type 1 diabetes. On day 4 of hyperglycemia, they were killed. C57BL6/KsJ db/db mice with type 2 diabetes were killed at 2 wk of hyperglycemia. Equal amounts of renal cortical nuclear extracts from control and diabetic rodents were separated by SDS-PAGE and immunoblotted with antibodies against phospho-UBF1, phospho-Erk, and phospho-p70S6 kinase (A-E). Immunoblotting with antibodies against lamin b, actin, and Erk was done to show purity of the nuclear preparation and assess loading. Each lane in the blot represents material from an individual animal, and composite data from control and diabetic rodents are shown in a histogram. Statistical significance between the two groups is shown as #P < 0.05 and †P < 0.01, by Student's t-test, diabetes vs. control.