Fig. 2.

Antioxidant tempol decreases age-related increase in NADPH-gp^91phox in the proximal tubular homogenate in old rats. NADPH-gp^91phox and β-actin were measured by Western blotting (details in methods). Results are means ± SE (n = 4–5 rats in each group). Top: representative blots of NADPH-gp^91phox and β-actin (loading control). Lane 1: adult control; lane 2: adult tempol; lane 3: old control; lane 4: old tempol. Bottom: bars are ratios of the densities between NADPH-gp^91phox and β-actin protein bands. ***Significantly different from adult rats at P < 0.001. ##Significantly different from old rats at P < 0.01 (1-way ANOVA followed by Newman-Keuls post hoc test).