Fig. 6.

Gal-3 expression alters matrix remodeling. A and B: representative Picrosirius red photomicrographs (×1,000) and corresponding polarized images (C, D) demonstrate increased intensity of red-orange collagen matrix in Gal-3-deficient mice on UUO day 14. E and F: representative confocal photomicrographs of Picrosirius red staining illustrates disordered interstitial matrix patterns (arrows) in Gal-3-deficient mice at day 14. G and H: representative 3-dimensional confocal image using Imaris 7.0 software illustrates vesicles-like structures containing Picrosirius red-positive matrix (arrows, G) in wild-type mice and confirmed disorganized interstitial matrix patterns in Gal-3-deficient mice (arrows, H). G, glomerulus. Magnifications are ×1,000 (A–D). Bar = 10 μm (E–H).