Fig. 6.
Induction of P2X7 expression in NRK-49F cells by cell supernatant. NRK-49F cells were treated with cell lysate prepared from different density of cells for 20 h (A) or treated with RPTC lysate from 6.6 × 105 for the indicated time (B). Cells were harvested and subjected to immunoblot analysis for the level of P2X7 and actin. The level of P2X7 was quantified by densitometry and normalized with actin (C and D). Representative immunoblots are from 3 or more experiments. Values are means ± SD of 3 independent experiments. Bars with different letters (a–d) are significantly different from one another (P < 0.05).