Fig. 7.

Effect of P2X₇ inhibition on cell supernatant-induced cell death in NRK-49F cells. NRK-49F cells were incubated with different concentration (0.5–2 μM) of A438079 for 1 h and then treated with cell supernatant for 20 h, and cell viability was assessed the MTT assay (A). Cells were treated with supernatant for 20 h in the presence or absence of 1 μM of A438079 and then harvested for immunoblot analysis for P2X7, active PARP, cleaved caspase-3, and actin (B). NRK-49F cells were transfected with siRNA targeting P2X7 or scrambled siRNA and allowed to grow for 24 h and then treated with supernatant for 20 h. Cell viability was assessed by the MTT assay (C), and cell lysates were subjected to immunoblot analysis for P2X₇, cleaved PARP, cleaved caspase-3, and actin (D). Values are means ± SD of 3 independent experiments. Bars with different letters (a–c) are significantly different from one another (P < 0.05).