Fig. 5.

Protein expression in heart homogenates. eNOS (A) and hypoxia-inducible factor-1α (HIF-1α; B) protein expression were greater (**P < 0.05) in ventricular homogenates from CIRKO-TAC vs. WT-TAC mice. HIF-1α was higher (*P < 0.05) in CIRKO-TAC vs. CIRKO-sham animals. HIF-1α was lower (**P < 0.05) in tissue from CIRKO-sham vs. WT-sham mice. Representative immunoblots are shown using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Results are means ± SE from 4 WT-sham, 5 WT-TAC, 7 CIRKO-sham, and 5 CIRKO-TAC mice.