Fig. 4.

Expression of short interfering (si)RNA directed against PTP1B rescues cells from the suppression of PKG induced by PDGF. Cells were cultured for 24 h in the presence of serum-free medium containing adenovirus expressing control siRNA or adenovirus expressing siRNA directed against PTP1B at a multiplicity of infection of 5–10 followed by a 24-h incubation in serum-free medium and 24 h in serum-free medium supplemented with 20 ng/ml PDGF plus virus expressing control siRNA or virus expressing siRNA directed against PTP1B. A: Western blots from a representative experiment. B and C: summary of results (means ± SE) from 3 experiments for PKG (B) and PTP1B (C), normalized to α-actin. *P < 0.05 compared with the control.