Fig. 5.

Overexpression of PTP1B, but not expression of dn-PTP1B, induces the suppression of PKG mRNA levels. Cells were cultured for 24 h in the presence of adenovirus expressing EGFP (control), PTP1B, or dn-PTP1B (C215S-PTP1B) at a multiplicity of infection of 5–10 followed by 24 h in serum-free medium. Real-time PCR was performed as described in materials and methods, and results were normalized to mRNA levels of cyclophilin D. Results are means ± SE of 6 experiments. *P < 0.05 compared with the control.