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. 2010 Nov 5;300(1):H57–H63. doi: 10.1152/ajpheart.00225.2010

Fig. 6.

Fig. 6.

Chronic treatment with a nitric oxide (NO) donor decreases PKG levels together with the upregulation of PTP1B levels and activity. Cells were cultured for 48 h in the presence of 50 or 100 μM diethylenetriamine NONOate (DETA-NO) followed by the measurement of protein levels via Western blot analysis. A: Western blots from a representative experiment. The gap between the lanes is because the control and DETA-NO lanes were not originally contiguous on the gel due to the splicing out of other data irrelevant to the presented results. B: summary of results (means ± SE) for PTP1B protein levels obtained from 3 experiments. *P < 0.05 compared with the control. C: summary of results (means ± SE) for PKG protein levels obtained from 3 experiments, normalized to α-actin levels. *P < 0.05 compared with the control. D: effect of DETA-NO on PTP1B activity. Results are means ± SE of 3 experiments, normalized to the control. *P < 0.05 compared with the control.